The Influence of Cryopreservation on Changes in Diameter and Compliance of Allografts in an Animal Experimental Model

Gabriel, M.; Wachal, K.; Dzieciuchowicz, L.; Pawlaczyk, K.; Krasiński, Z.; Oszkinis, G.

doi:10.1016/j.ejvs.2006.01.025

« Previous Next »European Journal of Vascular & Endovascular Surgery
Volume 32, Issue 2 , Pages 169-175, August 2006

The Influence of Cryopreservation on Changes in Diameter and Compliance of Allografts in an Animal Experimental Model

M. Gabriel
- Department of Vascular Surgery, University of Medical Science, Poznan, Poland
- Corresponding author. Marcin Gabriel, MD, PhD, Department of Vascular Surgery, University of Medical Science, ul. Dluga 1-2, 61-848 Poznan, Poland.
K. Wachal
- Department of Vascular Surgery, University of Medical Science, Poznan, Poland
L. Dzieciuchowicz
- Department of Vascular Surgery, University of Medical Science, Poznan, Poland
K. Pawlaczyk
- Department of Hypertension and Vascular and Internal Diseases, University of Medical Sciences, Poznan, Poland
Z. Krasiński
- Department of Vascular Surgery, University of Medical Science, Poznan, Poland
G. Oszkinis
- Department of Vascular Surgery, University of Medical Science, Poznan, Poland

Accepted 31 January 2006. published online 30 March 2006.

Abstract

Objective

The purpose of this study was to analyze the influence of cryopreservation on changes in diameter and compliance of allografts.

Methods

Sixty aortic allografts implanted in situ in rats were analyzed. The animals were divided into four groups that received fresh or cryopreserved isogenic (Lewis to Lewis) grafts, or fresh or cryopreserved allogenic (Lewis to DA) grafts, respectively. The diameter and compliance of the grafts were then visually evaluated with the digital video camera recorder after 15, 30, 60, 90 and 120 days.

Results

Gradual increase in diameter and decrease in compliance in case of all allogenic and cryopreserved isogenic grafts were observed. The observed changes in cryopreserved grafts were smaller when compared with fresh grafts, however, the differences did not reach statistical significance.