European Journal of Vascular & Endovascular Surgery
Volume 33, Issue 3 , Pages 330-339 , March 2007

Anti-endotoxin Hyperimmune Globulin Attenuates Portal Cytokinaemia, Phagocytic Cell Priming, and Acute Lung Injury after Lower Limb Ischaemia-reperfusion Injury

  • D.W. Harkin

      Affiliations

    • Regional Vascular Surgical Unit, The Royal Victoria Hospital, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland, UK
    • Division of Surgery and Perioperative Care, Queen's University of Belfast, School of Medicine and Dentistry, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland, UK
    • Corresponding Author InformationCorresponding author. Mr D.W. Harkin, Senior Lecturer in Surgery, Division of Surgery & Perioperative Care, Queen's University of Belfast, School of Medicine and Dentistry, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland, UK.
    • ,
  • R. Arnold

      Affiliations

    • Division of Surgery and Perioperative Care, Queen's University of Belfast, School of Medicine and Dentistry, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland, UK
    • ,
  • M. Hoper

      Affiliations

    • Division of Surgery and Perioperative Care, Queen's University of Belfast, School of Medicine and Dentistry, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland, UK

,Accepted 23 October 2006.

  • Image Result

    A: Portal endotoxin (pg/ml) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.009 compared with control. BP<0.05 compared with I/R group. B: Systemic endotoxin (pg/ml) in control, I/R, and anti

    A: Portal endotoxin (pg/ml) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.009 compared with control. BP<0.05 compared with I/R group. B: Systemic endotoxin (pg/ml) in control, I/R, and anti-LPS IgG+I/R groups. Data represents mean±SEM.

  • Image Result
    A: Portal TNF-alpha (pg/L) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.001 compared with control and anti-LPS IgG+I/R. BP<0.001 compared with I/R group. B: Systemic TNF-alpha (pg/L) in co

    A: Portal TNF-alpha (pg/L) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.001 compared with control and anti-LPS IgG+I/R. BP<0.001 compared with I/R group. B: Systemic TNF-alpha (pg/L) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.05 compared with control and anti-LPS IgG+I/R groups. Data represents mean±SEM.

  • Image Result
    A: Portal IL-6 (pg/L) in control, I/R, and rBPI21+I/R groups. AP<0.001 compared with control. BP<0.01 compared with I/R group. B: Systemic IL-6 (pg/L) in control, I/R, and anti-LPS IgG groups. A

    A: Portal IL-6 (pg/L) in control, I/R, and rBPI21+I/R groups. AP<0.001 compared with control. BP<0.01 compared with I/R group. B: Systemic IL-6 (pg/L) in control, I/R, and anti-LPS IgG groups. AP<0.003 compared with control. Data represents mean±SEM.

  • Image Result
    A: Portal phagocytic cell priming (% max.) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.01 compared with control. BP<0.05 compared with I/R group. B: Systemic phagocytic cell priming (% ma

    A: Portal phagocytic cell priming (% max.) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.01 compared with control. BP<0.05 compared with I/R group. B: Systemic phagocytic cell priming (% max.) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.01 compared with control. BP<0.05 compared with I/R group.

  • Image Result
    A: Arterial PO2 (mmHg) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.05 compared with control. BP<0.01 compared with I/R. B: Pulmonary artery pressure (PAP) in mmHg for control, I/R, and an

    A: Arterial PO2 (mmHg) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.05 compared with control. BP<0.01 compared with I/R. B: Pulmonary artery pressure (PAP) in mmHg for control, I/R, and anti-LPS IgG+I/R groups. AP<0.001 compared with control. BP<0.001 compared with I/R. C: (A-a) gradient in control, I/R, and anti-LPS IgG+I/R groups. AP<0.05 compared with control. Data represents mean±SEM. Repeated measures 2-way Anova (time and group), A: P<0.01, B: P<0.01, C: P<0.03. Post hoc Dunnett's T3 test, A: P<0.05, B: P<0.05, C: P<0.01.

  • Image Result
    A: Lung MPO (U/g) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.005 compared with control. B: BAL protein content (mg/L) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.05 compared with

    A: Lung MPO (U/g) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.005 compared with control. B: BAL protein content (mg/L) in control, I/R, and anti-LPS IgG+I/R groups. AP<0.05 compared with control. C: Lung W/D weight ratio in control, I/R, and anti-LPS IgG+I/R groups. AP<0.01 compared with control. Data represents mean±SEM.

 These studies were supported in part by a Wellcome Trust Project Grant, (DWH)

PII: S1078-5884(06)00612-5

doi: 10.1016/j.ejvs.2006.10.024

European Journal of Vascular & Endovascular Surgery
Volume 33, Issue 3 , Pages 330-339 , March 2007

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