European Journal of Vascular & Endovascular Surgery
Volume 17, Issue 1 , Pages 9-14 , January 1999

Transfection of Small Numbers of Human Endothelial Cells by Electroporation and Synthetic Amphiphiles

  • E.B.M van Leeuwen

      Affiliations

    • Centre for Biomedical Technology, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands
    • Department of Haematology, Division of Haemostasis, Thrombosis and Rheology, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands
    • ,
  • A.Y van der Veen

      Affiliations

    • Department of Medical Genetics, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands
    • ,
  • D Hoekstra

      Affiliations

    • Department of Physiological Chemistry, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands
    • ,
  • J.B.F.N Engberts

      Affiliations

    • Department of Organic and Molecular Inorganic Chemistry, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands
    • ,
  • M.R Halie

      Affiliations

    • Centre for Biomedical Technology, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands
    • Department of Haematology, Division of Haemostasis, Thrombosis and Rheology, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands
    • ,
  • J van der Meer

      Affiliations

    • Department of Haematology, Division of Haemostasis, Thrombosis and Rheology, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands
    • ,
  • M.H.J Ruiters

      Affiliations

    • Centre for Biomedical Technology, University of Groningen, Hanzeplein 1, GZ Groningen, 9713, The Netherlands

,Accepted 25 May 1998.

References 

  1. Powell JT, van Zonneveld AJ, van Mourik JA. Gene transfer into specific vascular cells. Eur J Vasc Surg. 1992;6:130–134
  2. Powell JT, Klaasse Bos JM, van Mourik JA. The uptake and expression of the factor VIII and reporter genes by vascular cells. FEBS Lett. 1992;303:173–177
  3. Fitzgerald U, McLean JS, MacDonald C. Production of immortal human endothelial cell lines by strontium phosphate transfection and electroporation of SV40 sequences. In Vitro Cell Dev Biol Anim. 1994;30A:236–242
  4. Nathwani AC, Gale KM, Pemberton KD, Crossman DC, Tuddenham EG, McVey JH. Efficient gene transfer into human umbilical vein endothelial cells allows functional analysis of the human tissue factor gene promoter. Br J Haematol. 1994;88:122–128
  5. Sipehia R, Martucci G. High-efficiency transformation of human endothelial cells by Apo E-mediated transfection with plasmid DNA. Biochem Biophys Res Commun. 1995;214:206–211
  6. Kirinaka H, Miyake K, Iijima S. Isolation of immortal human endothelial cells. Biosci Biotechnol Biochem. 1995;59:912–914
  7. Teifel M, Heine LT, Milbredt S, Friedl P. Optimization of transfection of human endothelial cells. Endothelium. 1997;5:21–35
  8. Laulajainen T, Julkunen I, Haltia A, Knuutila S, Miettinen A, Holthofer H. Establishment and characterization of a rat glomerular endothelial cell line. Lab Invest. 1993;69:183–192
  9. Rao CD, Pech M, Robbins KC, Aaronson SA. The 5′ untranslated sequence of the c-sis/platelet-derived growth factor 2 transcript is a potent translational inhibitor. Mol Cell Biol. 1988;8:284–292
  10. Fickling SA, Tooze JA, Whitley GS. Characterization of human umbilical vein endothelial cell lines produced by transfection with the early region of SV40. Exp Cell Res. 1992;201:517–521
  11. Kotnis RA, Thompson MM, Eady SL, Budd JS, Bell PR, James RF. Optimisation of gene transfer into vascular endothelial cells using electroporation. Eur J Vasc Endovasc Surg. 1995;9:71–79
  12. Kotnis RA, Thompson MM, Eady SL, Budd JS, James RF, Bell PR. Attachment, replication and thrombogenicity of genetically modified endothelial cells. Eur J Vasc Endovasc Surg. 1995;9:335–340
  13. van der Woude I, Wagenaar A, Meekel AA. Novel pyridinium surfactants for efficient, non-toxicin vitro. Proc Natl Acad Sci U.S.A. 1997;94:1160–1165
  14. van der Woude I, Visser HW, ter Beest MB. Parameters influencing the introduction of plasmid DNA into cells by the use of synthetic amphiphiles as a carrier system. Biochim Biophys Acta. 1995;1240:34–40
  15. Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973;52:2745–2756
  16. Mulder AB, Blom NR, Smit JW. Basal tissue factor expression in endothelial cell cultures is caused by contaminating smooth muscle cells. Reduction by using chymotrypsin instead of collagenase. Thromb Res. 1995;80:399–411
  17. Maciag T, Cerundolo J, Ilsley S, Kelley PR, Forand R. An endothelial cell growth factor from bovine hypothalamus: identification and partial characterization. Proc Natl Acad Sci U.S.A. 1979;76:5674–5678
  18. Gorman CM, Moffat LF, Howard BH. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982;2:1044–1051
  19. de Wet JR, Wood KV, DeLuca M, Helinski DR, Subramani S. Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol. 1987;7:725–737

PII: S1078-5884(98)90677-3

doi: 10.1053/ejvs.1998.0677

European Journal of Vascular & Endovascular Surgery
Volume 17, Issue 1 , Pages 9-14 , January 1999

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