European Journal of Vascular & Endovascular Surgery
Volume 17, Issue 1 , Pages 41-46, January 1999

Skeletal Muscle Tissue Oxygen Pressure Distribution During Early Reperfusion After Prolonged Ischaemia

  • U Gustafsson

      Affiliations

    • Clinical Research Center, Faculty of Health Sciences, University Hospital, Linköping, Sweden
    • ,
  • A Gidlöf

      Affiliations

    • Center for Molecular Medicine, Karolinska Institute at the Karolinska Hospital, Stockholm, Sweden
    • ,
  • B Povlsen

      Affiliations

    • Department of Orthopedics, St Thomas» Hospital, London, U.K.
    • ,
  • A Sirsjö

      Affiliations

    • Center for Molecular Medicine, Karolinska Institute at the Karolinska Hospital, Stockholm, Sweden

Accepted 16 June 1998.

Article Outline

Abstract 

Objectivesthe aim of this study was to investigate the skeletal muscle tissue oxygen pressure (PtO2) distributions during early reperfusion (10–45 min) after prolonged ischaemia in a rat animal model.Material and methodsskeletal muscle ischaemia was induced in anaesthetised rats by applying a tourniquet on the left thigh for 3 h (group I) or 4 h (group II), and tissue oxygen pressure measurements were made after 10–45 min of reperfusion. Assessment of PtO2was made by a multiwire Clark-type oxygen microelectrode, placed on the surface of the left tibialis anterior muscle.Resultsduring reperfusion a similar PtO2pattern was evaluated after both 3 and 4 h of total ischaemia, where the sum PtO2distributions were shifted to the left associated with low tissue oxygen pressure values. After 10 min of reperfusion the median PtO2was 0.28 kPa and 0.18 kPa, in groups I and II, respectively; after 45 min of reperfusion 0.61 kPa and 0.60 kPa, respectively. The median PtO2in the non-ischaemic muscle in groups I and II were 2.19 and 2.17 Pa.Conclusionthe results show that local skeletal muscle oxygenation is severely impaired during the initial 45 min of reperfusion after both 3 and 4 h of total muscle ischaemia with a slow-reflow phenomenon generally present, despite pronounced needs.

Keywords: ischaemia, reperfusion injury, skeletal muscle, tissue oxygenation.

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  • f1 Please address all correspondence to: A. Sirsjö, Cardiovascular Research Unite, Center for Molecular Medicine, L8:03 Karolinska Hospital, S-171 76 Stockholm, Sweden.

PII: S1078-5884(98)90693-1

doi:10.1053/ejvs.1998.0693

European Journal of Vascular & Endovascular Surgery
Volume 17, Issue 1 , Pages 41-46, January 1999

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