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Abstract
Introduction
This study aimed to examine the association of serum microRNAs (miRNAs) with diagnosis
and growth of abdominal aortic aneurysm (AAA), and to test their diagnostic and prognostic
value.
Methods
The expression levels of 800 miRNA tags were assessed in 108 AAA patients and 12 age-
and sex-matched healthy controls (HC) and 12 patients with peripheral artery disease
(PAD) using nanoString Technologies. Findings were assessed in an independent sample
of 66 AAA patients and 29 age and sex-matched HC by reverse transcriptase PCR (rtPCR).
AAA growth was assessed by a median of 3 (Interquartile range (IQR) 2, 3) repeat ultrasound
scans over a median follow up of 1.1 (IQR 1.0, 2.0) years. The association of the
miRNA with AAA diagnosis and growth were examined by regression and linear mixed effects
analyses. The diagnostic and prognostic potential of the miRNAs were examined using
area under the receiver operator characteristic curve (AUC), net reclassification
index (NRI) and Cox hazard analyses.
Results
In comparison to HC, a model combining clinical risk factors, let-7b-5p and miR-548n
had an AUC of 98.0% (95% CI 95.6, 100.0; p=.003) for diagnosing AAA, which was a significant
improvement over clinical risk factors alone (NRI: 1.74; 95% CI 1.61, 1.87; p<0.001).
In comparison to PAD, a model combining clinical risk factors and miR-548n had an
AUC of 99.6% (95% CI 98.9, 100.0, p=.037) for diagnosing AAA, which was a significant
improvement over clinical risk factors alone (NRI 1.79, 95% CI 1.68, 1.91; p<0.001).
In the longitudinal cohort, none of the miRNA were able to predict the likelihood
of reaching surgical threshold diameter better than clinical risk factors alone.
Conclusion
Serum let-7b-5p and miR548n significantly improved ability to diagnose AAA. None of
the miRNAs had independent prognosis value in predicting AAA growth.
Keywords
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Article info
Publication history
Accepted:
December 24,
2022
Received in revised form:
December 13,
2022
Received:
March 22,
2022
Publication stage
In Press Accepted ManuscriptIdentification
Copyright
© 2022 European Society for Vascular Surgery. Published by Elsevier B.V. All rights reserved.
