Advertisement

Association of serum microRNAs with abdominal aortic aneurysm diagnosis and growth

  • Author Footnotes
    1 Contributed equally.
    Shivshankar Thanigaimani
    Footnotes
    1 Contributed equally.
    Affiliations
    Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia

    Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns and Townsville, Queensland, Australia
    Search for articles by this author
  • Author Footnotes
    1 Contributed equally.
    Vikram Iyer
    Footnotes
    1 Contributed equally.
    Affiliations
    Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia

    Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns and Townsville, Queensland, Australia

    Department of Vascular Surgery, Royal Melbourne Hospital, Parkville, Victoria, Australia
    Search for articles by this author
  • John Bingley
    Affiliations
    Department of Vascular Surgery, Mater Hospital, Brisbane
    Search for articles by this author
  • Daniel Browne
    Affiliations
    Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns and Townsville, Queensland, Australia
    Search for articles by this author
  • James Phie
    Affiliations
    Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia

    Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns and Townsville, Queensland, Australia
    Search for articles by this author
  • Denise Doolan
    Affiliations
    Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns and Townsville, Queensland, Australia
    Search for articles by this author
  • Author Footnotes
    1 Contributed equally.
    Jonathan Golledge
    Correspondence
    Correspondence to: Professor Jonathan Golledge, Director, Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia, 4811; Fax +61 7 4796 1401 Telephone +61 7 4796 1417,
    Footnotes
    1 Contributed equally.
    Affiliations
    Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Queensland, Australia

    The Department of Vascular and Endovascular Surgery, Townsville University Hospital, Townsville, Queensland, Australia

    Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns and Townsville, Queensland, Australia
    Search for articles by this author
  • Author Footnotes
    1 Contributed equally.
Published:December 30, 2022DOI:https://doi.org/10.1016/j.ejvs.2022.12.028
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Introduction

      This study aimed to examine the association of serum microRNAs (miRNAs) with diagnosis and growth of abdominal aortic aneurysm (AAA), and to test their diagnostic and prognostic value.

      Methods

      The expression levels of 800 miRNA tags were assessed in 108 AAA patients and 12 age- and sex-matched healthy controls (HC) and 12 patients with peripheral artery disease (PAD) using nanoString Technologies. Findings were assessed in an independent sample of 66 AAA patients and 29 age and sex-matched HC by reverse transcriptase PCR (rtPCR). AAA growth was assessed by a median of 3 (Interquartile range (IQR) 2, 3) repeat ultrasound scans over a median follow up of 1.1 (IQR 1.0, 2.0) years. The association of the miRNA with AAA diagnosis and growth were examined by regression and linear mixed effects analyses. The diagnostic and prognostic potential of the miRNAs were examined using area under the receiver operator characteristic curve (AUC), net reclassification index (NRI) and Cox hazard analyses.

      Results

      In comparison to HC, a model combining clinical risk factors, let-7b-5p and miR-548n had an AUC of 98.0% (95% CI 95.6, 100.0; p=.003) for diagnosing AAA, which was a significant improvement over clinical risk factors alone (NRI: 1.74; 95% CI 1.61, 1.87; p<0.001). In comparison to PAD, a model combining clinical risk factors and miR-548n had an AUC of 99.6% (95% CI 98.9, 100.0, p=.037) for diagnosing AAA, which was a significant improvement over clinical risk factors alone (NRI 1.79, 95% CI 1.68, 1.91; p<0.001). In the longitudinal cohort, none of the miRNA were able to predict the likelihood of reaching surgical threshold diameter better than clinical risk factors alone.

      Conclusion

      Serum let-7b-5p and miR548n significantly improved ability to diagnose AAA. None of the miRNAs had independent prognosis value in predicting AAA growth.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to European Journal of Vascular and Endovascular Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect